Influence of high inlet pressure gas chromatography (HIPGC) on analytes retention time
Silva MR et al.
Palavras-chave: high pressure inlet gas chromatography; packed capillary column; gas chromatography.
Resumo: High inlet pressure gas chromatography (HIPGC) is an analytical tool that offers several advantages including high efficiency, fast analysis time, and easier elution of high molecular mass compounds. In this paper a basic study regarding the chromatographic performance of packed capillary columns (length to 25 cm) utilizing a modified commercial GC instrument was carried out. This lab-made GC system consisted in a new device connected to the GC injector aiming the application of gas chromatography in two different modes: low inlet pressure and high inlet pressure (c.a. 2000 psi). The inlet pressure was varied between 500 – 2000 psi and the influence of this pressure transition in the chromatographic efficiency was evaluated being compared with other GC approaches. The obtained results applying this modified GC instrument shows that increasing the inlet pressure is possible to reduce the analysis time as well as improve the analytes signal. In addition, this work shows the use of packed capillary columns packed with standard HPLC stationary phases as an alternative approach to GC analysis without requiring temperature programming.
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