http://dx.doi.org/10.5935/sc.2018.006

 

Extração líquido-líquido assistida pelo efeito salting out para análise de amostras  biológicas

Grecco CF et al.

Palavras-chave: extração líquido-líquido assistida pelo efeito salting-out; preparo de amostra biológicas.

ResumoA extração líquido-líquido assistida pelo efeito salting out (SALLE, Salting-out assisted liquid–liquid extraction) é baseada na partição do(s) analito(s) entre o solvente orgânico miscível em água e amostras de plasma ou outros fluidos biológicos. O efeito salting-out favorece a separação entre as fases orgânica e aquosa. A SALLE é uma técnica de preparo de amostra que possibilita a extração de substâncias hidrofílicas, incluindo fármacos e seus metabólitos. Esta técnica possibilita que os extratos obtidos sejam injetados diretamente no sistema cromatográfico, ou após simples diluição. Esta revisão apresenta os fundamentos teóricos da técnica SALLE, as principais etapas envolvidas no processo de extração e exemplos de aplicações desta técnica em diversas áreas.


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