http://dx.doi.org/10.5935/sc.2019.001

Métodos analíticos para determinação de isoflavonas em matrizes complexas

Silva L. F., Sinisterra M. J., Lanças F. M.

Palavras-chave: isoflavonas, matrizes complexas, métodos analíticos, preparo de amostra, técnicas de separação.

Resumo: As isoflavonas formam uma importante subclasse dos fitoestrógenos. Encontradas em todo o reino vegetal, estão diariamente presentes em nossa alimentação e são associadas a diversos benefícios à saúde humana, como prevenção de doenças cardiovasculares e de câncer de mama. Esses fatores despertaram o interesse da comunidade científica, ocasionando um expressivo aumento no número de publicações relacionadas a estes compostos nas últimas duas décadas. Este trabalho traz uma visão geral das técnicas de preparo de amostra; técnicas cromatográficas e eletroforéticas de separação; e técnicas de detecção acopladas, assim como suas aplicações em análises de isoflavonas nas mais diversas matrizes, desde 2010 até 2018. São relatados trabalhos de análises de isoflavonas em soja, produtos comerciais derivados de soja, leite bovino, urina, plasma, água e outros. Dentre as técnicas de preparo de amostra destacam-se os trabalhos com LLE, SLE, SPE, QuEChERS, MSPD SPME e MEPS. O uso de técnicas clássicas de preparo de amostra ainda é o mais comum; mesmo com micro técnicas já bem desenvolvidas como a SPME e a MEPS, o uso destas ainda é escasso limitando-se, principalmente, à trabalhos de desenvolvimento analítico. Dentre as técnicas de separação, as cromatográfica, como a HPLC, UHPLC e GC são destaque; as técnicas eletroforéticas também encontram aplicações na área. Dentre os detectores mais empregados estão os espectrômetros massas e os espectrofotômetros na região UV-Vis, respectivamente.


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