http://dx.doi.org/10.5935/sc.2019.008

Enantioseparation of underivatized amino acids by capillary liquid chromatography. 2. (N, S-dioctyl-(D)-penicillamine) ligand exchange chiral stationary phase

Daher F. A., Santos N. G. P., Maciel E. V. S., Lanças F. M.

Palavras chaves: Separação enantiomérica, cromatografia líquida capilar, fase estacionária quiral, troca de ligantes, aminoácidos, complexos de Cobre(II)

Resumo: A cromatografia líquida quiral capilar de aminoácidos intactos (não derivatizados) foi investigada usando uma fase estacionária a qual atua através da troca de ligantes (N, S-dioctyl-(D)-penicillamine), e uma fase móvel contendo íons Cu(II) como agente complexante. Uma separação direta de quatro α-amino ácidos racêmicos foi obtida, e a influência das variáveis experimentais foi investigada. A separação ocorreu no modo isocrático a um fluxo de 8µL/min, com detecção UV em 254 nm. Os resultados mostraram que a cromatografia líquida capilar empregando Cu(II) na fase móvel é uma boa alternative para a separação enantiomérica de amino ácidos quirais intatos.


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