v1n3a5en

Vantagens e limitações da miniaturização em cromatografia líquida

Lanças, Fernando M.

Palavras-chave: Capillary liquid chromatography, miniaturization, HPLC, micro-LC.

Resumo: In spite of the several advantages we will obtain by employing capillary columns instead of the conventional HPLC columns in liquid chromatography, this miniaturization have not obtained the same popularity as that obtained by its counterpart in gas chromatography, meaning HRGC (“High Resolution Gas Chromatography”). In the present work, the main advantages of using capillary LC columns are introduced and critically discussed, as well as the major facts that still hinder the full development of this technique.

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1. M. Tswett, Physikalisch-chemische Studien Über das Chlorophyll. Die Adsorptionen., Ber. Dtsch. Botan. Ges. 24, 316-326 (1906)

2. M. Tswett, Adsorptionsanalyse und chromatographische Methode. Anwendung auf die Chemie des Chlorophylls., Ber. Dtsch. Botan. Ges. 24, 384-392 (1906)

3. A.J.P. Martin and R.L.M. Synge, Biochem. J. 35, 91 (1941).

4. A.J.P. Martin and R.L.M. Synge, Biochem. J. 35, 1358–1368 (1941).

5. A.T. James and A.J.P. Martin, Biochem. J. 50, 679–690 (1952).

6. M.J.E. Golay, Anal. Chem. 29, 928–932 (1957).

7. M.J.E. Golay, “Discussion of the Results With Three Experimental Gas-Liquid Chromatographic Columns”. Engineering Report No. 523, Perkin-Elmer Corp. (Norwalk, Connecticut, 5 September 1956).

8. M.J.E. Golay, in Gas Chromatography (1958 Amsterdam Symposium), D.H. Desty, Ed. (Butterworths,London, 1958), pp. 36–55.

9. F.M. Lanças, “Cromatografia Líquida de Alta Eficiência”, Ed. Átomo, 2009.

10. T. Takeushi, Chromatography 26, 1 (2005).

11. R.D. Dandeneau and E.H. Zerenner, HRC &CC 1, 351–356 (1979)

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