Problemas com o formato dos picos em cromatografia líquida

Santos Neto, Álvaro José

Palavras-chave: Asymmetry, peak tailing, peak fronting, peak distortions, HPLC, liquid chromatography.

Resumo: This edition continues discussions about peak shape problems occurring in liquid chromatography. Peak distortions like tailing, fronting, widening, and double peaks or peaks with shoulders may occur due to several reasons and this matter needs to be detailed to provide chromatographic users with background to solve these problems. Following the last article, this one brings not only physical but also chemical aspects related with separation. The most common contributions to peak distortion will be briefly studied and exemplified for a better understanding, and strategies to overcome these problems will be presented as well.

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Referências Bibliográficas

1. Scott, R.P.W., Chromatography Books Series: Preparative Chromatography. Disponível online: , Acesso em: 02 nov. 2009.

2. Santos-Neto, A.J. Cromatografia líquida multidimensional e espectrometria de massas em tandem para análise direta de fármacos em fluidos biológicos: da escala convencional à miniaturizada.

2007. 216 f. Tese de Doutorado – Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos,

2007. Disponível em: Acesso em: 02 nov. 2009.

3. Meyer, V.R. High-performance liquid chromatographic theory for the practitioner. J. Chromatogr., v. 334, p. 197-209, 1985.

4. Kucera, P. Microcolumn high-performance liquid chromatography. Amsterdam: Elsevier, 1984 (Journal of chromatography library 28)