Basic concepts in capillary electrophoresis

Spudeit, Daniel Alfonso; Dolzan, Maressa Danielli; Micke, Gustavo Amadeu

Palavras-chave: Capillary Electrophoresis, electroosmotic flow, instrumentation, injection modes.

Resumo:  Historically, the Swedish chemist Arne Tiselius was the first to use Electrophoresis as a separation tool, applying in blood proteins. The Capillary Electrophoresis emerged after decades as an improved technique providing an increase in efficiency and reduced analysis time. Its applications are wide and the range is from small and simple molecules organic and inorganic, to large and complex, as protein molecules and nucleic acids. This peculiar versatility of Capillary Electrophoresis makes it a promising tool for several knowledge areas. The methodologies developed by this technique can be improved offline by optimization and simulation softwares, highlighting the Peakmaster® software. This present paper will further introduce Capillary Electrophoresis instrumental aspects, injection modes and detection, as well as peculiar phenomena of technique, as electroosmotic flow.

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