Determination of herbicide residues in rice paddy water by solid phase extraction and high performance liquid chromatography with diode array detection

Gonçalves, Fabio Ferreira; Matos, Fabio da Silva de; Zanella, Renato

Palavras-chave: Pesticides, surface water, SPE, HPLC-DAD.

Resumo: The herbicides used on crops are generally persistent and can pollute water supplies. Therefore, this study aimed to develop and validate a method for the determination of residues of the herbicides clomazone, imazethapyr and imazapic in rice paddy water. The method involves sample preparation using Solid Phase Extraction, to allow an appropriate concentration factor, and quantification by High Performance Liquid Chromatography with Diode Array Detection. After optimization of the extraction and analysis parameters, the method was validated by evaluating analytical curve, linearity, limits of detection and quantification, precision (repeatability and intermediate precision) and accuracy. The results showed excellent linearity for all compounds (r2 > 0.99) in the range between the limit of quantification and 2.5 mg L–1. The method showed recoveries between 81 and 107%, with RSD < 9%, and was applied for the determination of residues of herbicides in rice paddy water with limits of quantification (LOQ) of 0.12 μg L–1 for imazapic and imazethapyr, and of 0.25 μg L–1 for clomazone.

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