Técnicas cromatográficas e o desafio na determinação de compostos presentes em matrizes complexas: Relato de um caso prático do produto natural piperlongumina

Marques, Lucas Maciel M.; Oliveira, Anderson Rodrigo M. de

Palavras-chave: CLAE, CLUE, CG, produto natural.

Resumo: A elevada diversidade química dos produtos naturais de plantas representa um desafio para os métodos analíticos de separação e detecção, sendo difícil empregar um procedimento único de análise para todos os compostos presentes em um extrato. Atualmente, métodos cromatográficos como a CLAE, a CLUE e a CG têm desempenhado um importante papel quanto à eficiência de separação. A vantagem de cada uma dessas técnicas dependerá do propósito experimental requerido. No presente trabalho analisar-se-ão as vantagens e desvantagens de cada uma dessas técnicas cromatográficas na análise do(s) metabólitos(s) do produto natural piperlongumina, após essa ser metabolizada por microssomas de fígado de ratos. Mediante as comparações, conclui-se que as técnicas não se excluem e não são competitivas, pelo contrário, são complementares, cada uma com suas especificidades e limitações.

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