http://dx.doi.org/10.4322/sc.2015.019
Extração em ponteiras descartáveis: fundamentos teóricos e aplicações
Pinto, Mônia Aparecida Lemos; Queiroz, Maria Eugênia C.
Palavras-chave: Extração em ponteiras descartáveis, preparo de amostra.
Resumo: A extração em ponteiras descartáveis – Diposable Pipette Extraction (DPX), recente técnica de preparo de amostra, é baseada no equilíbrio de sorção do analito presente na amostra com o sorvente. Esta técnica consiste em uma variante da extração em fase sólida (SPE) convencional. A DPX utiliza uma ponteira convencional de pipeta (1 ou 5 mL), na qual o sorvente está contido livremente entre dois filtros. A amostra aspirada pela ponteira é misturada dinamicamente com o sorvente por meio da aspiração de ar. A extração ocorre em fase sólida dispersiva, assegurando adequada superfície de contato (amostra/sorvente) e conseqüentemente, eficiente extração. A DPX é uma técnica de preparo de amostra rápida e simples que utiliza reduzido volume de amostra e de solvente orgânico. Esta revisão apresenta os fundamentos teóricos da técnica DPX, as principais etapas envolvidas no processo de extração e exemplos de aplicações desta técnica de preparo de amostra, nas áreas de toxicologia forense, ambiental e clínica.
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