http://dx.doi.org/10.4322/sc.2015.019
Disposable Pipette Extraction (DPX): fundamental principles and applications
Pinto, Mônia Aparecida Lemos; Queiroz, Maria Eugênia C.
Palavras-chave: Disposable Pipette Extraction, sample preparation.
Resumo: Disposable Pipette Extraction (DPX), recent technique of sample preparation, is based on analyte sorption equilibrium between the samples and sorbent. This technique consists of a variant of conventional solid phase extraction (SPE). DPX uses a standard pipette tip (1 or 5 ml), in which the sorbent is contained loosely between two filters. Suction of air promotes dynamically mix of the sample and the sorbent inside the tip. The extraction occurs in dispersive solid phase, ensuring adequate contact surface, and consequently, efficient extraction. The DPX is a fast and simple sample preparation technique that uses small sample volume and the organic solvent. This review presents the theory fundamentals of DPX technique, the key steps involved in the extraction process and examples of applications in the areas of forensic toxicology, environmental and clinical.
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