http://dx.doi.org/10.4322/sc.2017.003

 

Análisis por GC/FID y GC/MS de la composición química y estudio de la actividad antioxidante de los metabolitos secundarios volátiles, aislados por diferentes técnicas, de Satureja viminea L. cultivada en Colombia

Stashenko, Elena E.; Gutiérrez-Avella, Dora M.; Martínez, Jairo René; Manrique-López, Diana Lisseth

Palavras-chave: Satureja viminea L., aceite esencial, MWHD, SDE, SFE-CO2 , HS-SPME, GC/FID, GC/MS, actividad antioxidante.

Resumo: Los metabolitos secundarios volátiles de Satureja viminea L. se aislaron por diferentes técnicas, i.e., hidrodestilación asistida por microondas (MWHD), destilación-extracción simultánea con solvente (SDE), extracción con fluido supercrítico (SFE-CO2 ), y microextracción en fase sólida de espacio de cabeza (HS-SPME). La identificación de los componentes se realizó por GC-MS y su cuantificación por GC-FID, usando n-tetradecano como estándar interno (Istd). Los monoterpenos oxigenados p-ment- 3-en-8-ol (51,2%) y pulegona (17,8%) fueron los compuestos mayoritarios del aceite esencial, mientras que en los extractos, obtenidos por SDE y SFE-CO2 , el componente mayoritario, p-ment-3-en-8-ol, alcanzó 58.0% y 38.9%, respectivamente. En la fracción volátil de la planta, aislada por HS-SPME, predominaron p-ment-3-en-8-ol (46,2%), pulegona (11,2%) y pulegol (5,9%). La actividad antioxidante del aceite esencial, determinada por diferentes métodos (ensayos ORAC, ABTS+• y monitoreo de la peroxidación lipídica), en general, fue más baja que la de las sustancias de referencia, i.e., α-tocoferol (vitamina E) y 2,6-di-tert-butil-4-metilfenol (BHT), excepto la determinada por el ensayo ORAC, cuyo valor (1060 ± 16 mmol Trolox® /g sustancia) era casi dos veces más alto que la de las sustancias de referencia (vitamina E o BHT).


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