v9n2doi10.4322/sc.2017.010 | Scientia Chromatographica

http://dx.doi.org/10.4322/sc.2017.010

Avanços recentes na miniaturização de colunas para cromatografia líquida

Leal, Vivane Lopes; Silva, Meire Ribeiro da; Lanças, Fernando Mauro

Palavras-chave: Colunas miniaturizadas: monolítica, tubular aberta, empacotada, micro chip; cLC.

Resumo: Nas diversas técnicas cromatográficas, os avanços da instrumentação e da tecnologia de preparo de colunas têm conduzido, cada vez mais, a análises mais rápidas com aumento no poder de separação. Por sua vez, a miniaturização vem ganhando espaço e demonstrando ser uma tendência nesta área, nos últimos anos. No caso da cromatografia liquida miniaturizada as vantagens estão concentradas, principalmente, no menor consumo de amostra, sorvente e fase móvel, assim como na baixa vazão de fase móvel a qual eleva a relação sinal/ruído dos detectores sensíveis à concentração do analito sendo detectado. Neste contexto, ocorre a diminuição das dimensões das colunas, surgindo a necessidade de obter-se colunas com elevada eficiência o que, em conjunto com o sistema miniaturizado de cromatografia liquida, torna possível a exploração de diferentes tipos de colunas miniaturizadas como as microempacotadas, as monolíticas, tubulares abertas e as colunas preparadas no formato de microchips, as quais possuem ótimo potencial para serem exploradas em cromatografia líquida tanto em escala micro (micro-LC) quanto em escala nano (nano- LC). Essas colunas serão descritas e avaliadas no presente trabalho.

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