http://dx.doi.org/10.5935/sc.2018.003

 

Capillary UHPLC: theoretical and practical aspects

Marcio David Bocelli MD; Borsatto JVB; Santos Neto AJ

Palavras-chave: Capillary UHPLC, packed columns, frictional heating, wall effect, capillary columns.

Resumo: Capillary UHPLC is a very interesting tool. It combines the main characteristics of UHPLC and capillary liquid chromatography, such as the capability to work with small amounts of sample as well as rapid and efficient analysis. Although this technique presents interesting characteristics, it is not present in a large number of publications, being restricted, basically, to chromatographic separations in the area of proteomics. This review presents the main theoretical and practical aspects about capillary UHPLC. Relevant fundamentals such as particle diameter effect, column inner diameter effect, pressure effect, fictional heating and wall effect are concisely described. A historical approach is presented on the evolution of UHPLC and the columns miniaturization. The main features of instrumentation are also addressed


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