https://dx.doi.org/10.5935/sc.2018.006
Salting-out assisted liquid-liquid extracion for biological sample analysis
Grecco CF et al.
Palavras-chave: Salting-out assisted liquid–liquid extraction; biological sample preparation.
Resumo: Salting-out assisted liquid-liquid extraction (SALLE) extraction is based on the partition of analyte(s) between water-miscible organic solvent and plasma samples or other biological fluids. The salting-outeffect induces the separation between the phases, organic and aqueous. SALLE is a sample preparation technique that enables the extraction of drugs and their metabolites, including many hydrophilic compounds. This technique allows the obtained extracts to be directly injected into the chromatographic system or by simple dilution. This review presents the theory fundamentals of SALLE technique, the key steps involved in the extraction process and examples of applications of this technique in several areas.
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