Comentários desativados em v11n4doi10.5935/sc.2019.013en

v11n4doi10.5935/sc.2019.013en

http://dx.doi.org/10.5935/sc.2019.013

GC-MS/MS versus LC-MS/MS for unambiguous identification of α and β-trenbolone in bovine urine

Silveira A. L., Faria A. F.

Keywords: bovine urine, gas chromatography tandem mass spectrometry, liquid chromatography tandem mass spectrometry, microwave assisted derivatization, trenbolone.

Abstract: This work involves the optimization of LC-MS/MS and GC-MS/MS methods for determination of α and β-trenbolone in bovine urine. The parameters recovery, repeatability and intermediate precision in both methods met the acceptance criteria of the Codex Alimentarius: recovery between 83.5-114% and repeatability and intermediate precision with RSD between 5.1-14.5% and 9.8-25.5% respectively, for concentrations higher than 1 μg kg−1. These methods were applied to the analyses of 5 real samples suspected of contamination. The analyses showed that derivatization with MSTFA/I2 followed by pure MSTFA, was an important tool to prevent false positive results. The use of microwave assisted derivatization reduced significantly the analysis time by GC-MS/MS.

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