Extração sortiva em barra de agitação (SBSE): Fundamentos teóricos e fases seletivas
Queiroz, Maria Eugênia C.
Palavras-chave: Stir bar sorptive extraction, SBSE process, thermal desorption, liquid desorption, selective phases.
Resumo: Stir Bar Sorptive Extraction (SBSE) is a sample preparation technique that integrates the sample extraction and solute concentration in one step, employing a re-utilizable probe. After all, the stir bar containing the concentrated solute(s) is introduced in a chromatograph, decreasing solute losses and analysis time. The SBSE efficiency is related to the partition coefficient of the solute between the phases, the hydrophobic character of the solute (logarithm of the octanol-water partition coefficient), the sample volume, and the stir bar dimensions. PDMS (non-polar) is the only SBSE phase available in the market. In this article, we describe the fundamental theory, the SBSE process, thermal desorption, liquid desorption, as well as the development and application of new lab-made SBSE bars. These bars are coated with phases that are more selective for polar compounds (such as PDMS/b-cyclodextrine, poliurethane, PDMS/adsorvent material); restrict access medium (RAM) material containing both octadecilsiloxane (C-18) and macromolecules showing exclusion behavior (hydrophilic biocompatible surface, diol); and mixed phases (PDMS/adsorbent and PDMS/polypyrrole) where the extraction is based on adsorption and absorption process, favoring the sensibility and selectivity enhancement of the analytical method.b-cyclodextrine, poliurethane, PDMS/adsorvent material); restrict access medium (RAM) material containing both octadecilsiloxane (C-18) and macromolecules showing exclusion behavior (hydrophilic biocompatible surface, diol); and mixed phases (PDMS/adsorbent and PDMS/polypyrrole) where the extraction is based on adsorption and absorption process, favoring the sensibility and selectivity enhancement of the analytical method.
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