Microextração em fase sólida no capilar (in-tube SPME) para automação das análises de fármacos em fluidos biológicos
Queiroz, Maria Eugênia C.
Palavras-chave: In-tube solid-phase microextraction, drugs, biological samples.
Resumo: The in-tube SPME system developed in combination with the liquid chromatography (LC) has been used for the automation of analyses SPME/LC of less volatile and/or thermally unstable solutes. The system in-tube SPME/LC can be done by fixing an open silica capillary column, internally coated with the phase stationary (or a capillary column gas chromatography), between the injection loop and injection needle of the LC autosampler, or simply, substituting the injection loop. The technique in-tube SPME/LC, when compared with the conventional SPME, minimizes the sample volume, allows automation of the chromatographic methods by means of extraction and concentration of the solutes on-line with separation and chromatographic detection that results in better analytical accuracy and precision, and short analysis time. As the samples are injected in the system, practically in its physiological state, decreases the exposition of the analysts to biological fluids. The development of new in-tube SPME phases increases the ability and the potential for in-tube SPME. Molecularly imprinted polymer, monolithic and imunoaffinity phases allowed high analytical selectivity/specificity, biocompatible RP-18/diol, the direct extraction of the drugs with exclusion of proteins, b-cyclodextrin (b-CD), interactions by inclusion complexes between drugs and b-CD, and polypirrole, high permeability and acid-base intermolecular interactions.
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