Técnicas de pré-concentração on line em eletroforese capilar

Bonato, Pierina Sueli

Palavras-chave: Capillary electrophoresis, on line pre-concentration, stacking.

Resumo: In order to increase the detectability of capillary electrophoresis methods, several on line pre-concentration techniques have been developed in recent years. These techniques, grouped in four different classes (stacking, dynamic pH junction, transient isotachophoresis and sweeping), make possible to increase the sample volume introduced in the capillary without loss of efficiency and resolution. As a result, detection limits and quantification limits similar to those obtained using other techniques such as high-performance liquid chromatography can be obtained. In this report, the fundamental aspects of these on line pre-concentration techniques are discussed.

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