Increasing the efficiency of HPLC columns by decreasing the particles diameter: till where?

Lanças, Fernando M.

Palavras-chave: HPLC, U-HPLC, microparticles, column efficiency .

Resumo:  Since the development of instrumental modern liquid chromatography (HPLC), very few was done during the first two decades in order to extend the possibilities of the technique. During the third decade an effort was done aiming the development of smaller, more uniform, and more reproducible particles to increase the efficiency of the columns and, as a consequence, the chromatographic resolution. However, the dramatic reduction in the particles size – from 10 to 1.7 μm – produced a considerable increase in the column backpressure, achieving values above those supported by conventional HPLC instruments In the present work we discuss the lowest limit size to which the particles should decrease, as well as if this is the best way to increase the chromatographic resolution.

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