Capillary liquid chromatography: 2. Band broadening due to extracolumn effects

Coutinho, Lincoln F. M.; Lanças, Fernando M.

Palavras-chave: Capillary liquid chromatography, band broadening, extracolumn effects, micro-LC, capillary LC.

ResumoBand broadening is one of the major factors to be avoided in chromatographic techniques, once it decreases the column efficiency (the number of plates, N) and deteriorates the Resolution (Rs). This effect is particularly critical in capillary liquid chromatography due to the very low volumes, instrumentation, connections and columns employed in this technique. As a part in a series of papers dealing with c-LC, this work describes the major factors responsible for band broadening in chromatography, as well as the ways to minimize it.

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