Temperature effects in high performance liquid chromatography (HPLC)

Lanças, Fernando M.

Palavras-chave: High Temperature Liquid Chromatography, HTLC, Elevated Temperature Liquid Chromatography (ETLC), ETLC, Temperature effects in HPLC.

Resumo:  For several decades the major characteristics to clearly diferentiate gas chromatography (GC) from liquid chromatography (LC) has been the fact that most GC separation occurred at elevated temperatures while LC usually has been performed at room temperature. However, upon the development of chemically bonded phases (ex. RP-18), more temperature dependent separation mechanisms started to be used, and the temperature control became an important issue to control the retention times. More recently it was observed that the use of elevated temperatures in LC broughth several advantages over the same separations performed at room temperature thus starting a new area termed ETLC (Elevated Temperature Liquid Chromatography) or HTLC (High Temperature Liquid Chromatography). This paper presents an overview on the role of the temperature in LC, in special about its influence on both selectivity and retention.

Referências Bibliográficas

1. Teutenberg T. Potential of high temperature liquid chromatography for the improvement of separation efficiency – A review. Analytica Chimica Acta 2009; 643:1-12. PMid:19446057. http://dx.doi. org/10.1016/j.aca.2009.04.008

2. Yang Y. Subcritical water chromatography: A green approach to high-temperature liquid chromatography. Journal of Separation Science 2007; 30(8):1131- 1140.. PMid:17595948. http://dx.doi.org/10.1002/ jssc.200700008

3. Teutenberg T, Tuerk J, Holzhauser M, Giegold S. Temperature stability of reversed phase and normal phase stationary phases under aqueous conditions. Journal of Separation Science 2007; 30(8):1101- 1114.. PMid:17595945. http://dx.doi.org/10.1002/ jssc.200600507

4. Teutenberg T. High temperature liquid chromatography – A brief review about an emerging technique. Chromatography Today 2010; 3(3):3-6.

5. Zhun C, Goodall D, Wren S. Elevated temperature HPLC: principles and applications to small molecules and biomolecules. LCGC Asia Pacific 2005; 8(1):48-59.

6. Lundanes E, Greibrokk T. Temperature effects in liquid chromatography. Advances in Chromatography 2006.; 2:45-77.

7. Yang Y. A model for temperature effect on column efficiency in high-temperature liquid chromatography. Analytica Chimica Acta 2006; 558:7-10. PMid:17386578. http://dx.doi.org/10.1016/j.aca.2005.11.011

8. Vanhoenacker G. Elevated temperature and temperature programming in conventional liquid chromatography – fundamentals and applications. Journal of Separation Science 2006; 29(12):1822-1835. PMid:16970186. http://dx.doi.org/10.1002/jssc.200600160

9. Jones A, Marin S, Clark J, Porter N. In: Proceedings of the XXIX International Symposium on High Performance Liquid Phase Separations and Related Techniques; 2005; Stockholm. Stockholm; 2005. p. 3-30.

10. Yang Y. Stationary Phases for LC Separations at Elevated Temperatures. LCGC North America 2006; 24(S4):53-58.