Temperature effects in high performance liquid chromatography (HPLC)

Lanças, Fernando M.

Palavras-chave: High Temperature Liquid Chromatography, HTLC, Elevated Temperature Liquid Chromatography (ETLC), ETLC, Temperature effects in HPLC.

Resumo For several decades the major characteristics to clearly diferentiate gas chromatography (GC) from liquid chromatography (LC) has been the fact that most GC separation occurred at elevated temperatures while LC usually has been performed at room temperature. However, upon the development of chemically bonded phases (ex. RP-18), more temperature dependent separation mechanisms started to be used, and the temperature control became an important issue to control the retention times. More recently it was observed that the use of elevated temperatures in LC broughth several advantages over the same separations performed at room temperature thus starting a new area termed ETLC (Elevated Temperature Liquid Chromatography) or HTLC (High Temperature Liquid Chromatography). This paper presents an overview on the role of the temperature in LC, in special about its influence on both selectivity and retention.

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