UHPLC – The present situation: developments and recent challenges

Maldaner, Liane; Jardim, Isabel Cristina S. F.

Palavras-chave: UHPLC, stationary phases, instrumentation, UHPLC × HPLC.

Resumo Ultra-high performance liquid chromatography (UHPLC) emerged with the advent of sub-2 μm porous particles catalyzed by the continuous search for faster and more efficient analyses, and retains the same separation principles as high performance liquid chromatography (HPLC). Since its introduction in 2004, UHPLC has grown in all HPLC separation areas as a consequence of its advantages and, thus, has been a driving force for new research, especially in terms of novel stationary phases and improved equipment. The main challenges for using UHPLC, in comparison with HPLC, have already been identified and will be discussed in this paper together with recent developments in both stationary phases and instrumentation.

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