Modern liquid chromatography and mass spectrometry: Finaly compatible? II. Selecting the mass analyzer

Lanças, Fernando M.

Palavras-chave: Liquid Chromatography, HPLC, CLAE, Mass Spectrometry, LC/MS.

Resumo: High Performance Liquid Chromatography (HPLC) is one of the most utilized analytical techniques for determination of nonvolatile and thermally unstable compounds. Although being an excellent separation technique, HPLC requires a confirmatory technique when qualitative analysis is being performed. Amongst the several available complimentary techniques, Mass Spectrometry is the one that supplies the structural information required to complement the HPLC separation; by coupling these two techniques, a powerful tool become available for qualitative and quantitative analysis: LC/MS (Liquid Chromatography-Mass Spectrometry). This paper, the second one in a series, presents a critical and detailed discussion on the proper selection of the ion analyzer for LC-MS(MS).

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