Development of a new liquid phase microextraction method with hollow fiber HF-SBME for the analysis of the organochlorine compounds in water samples by GC-ECD

Ladino, Jhon Alexander Fiscal; Chacón, Sandra Liliana Correa; Loaiza, Sandra Ceballos; Salcedo, Alberto de la Ossa; Ocampo, Gonzalo Taborda; Nerin, Cristina

Palavras-chave: Organochlorine compounds, liquid phase microextraction, HF-SBME, CG‑ECD, pesticide, leachate, drinking water.

Resumo: A novel liquid phase microextraction (LPME) method has been developed, by coating with hollow fiber a metallic paper pin. Into of lumen of 30 mm of accurel membrane (300/1200 PP® Membrana GmbH Wuppertal-Germany) was put a 10 mm metallic piece, functionalizing the hollow fiber as a solvent bar and was used for the organochlorine compounds extraction from several aqueous samples.This new technique was coined as hollow fiber solvent bar microextraction HF-SBME, and has taken the advantages of the liquid phase microextraction with membrane such as: to serve as barrier for interferences, has a high enrichment factor, cheap and disposable to avoid carry over for one side and the skills of the stir bar sortion extraction such as: a big surface contact, a big mass transference capability and great facilities to recover the bar for the other side. The optimal conditions for the new microextraction method were: 1-octanol as organic solvent filled into membranes´s lumen (mode two phases); 20 min as extraction time; 700 rpm as stirring speed; 30 °C as temperature extraction; 20% NaCl as salting out effect. Under these conditions and after extraction procedure was injected 1 µL of extracted solvent from membrane´s lumen with Hamilton 10 µL syringe, and used a gas chromatography equipped with electron capture detector GC-ECD for the analysis of six organochlorine (except carbaryl) pesticide (endrin, dieldrin, 4,4´-DDD, aldrin, lindane) compounds in several aqueous samples as a leachate landfill and drinking water.

Referências Bibliográficas

1. Rosero-Moreano M., et al. 2012. Water Air and Soil Pollution, 223, 2, 667-678.
2. Rosero-Moreano M., et al 2011. J. Braz. Chem. Soc., 22, 12, 2230-2236
3. Anon Directiva 2000/60 / CE del Parlamento Europeo y del Consejo, de 23 de octubre que establece un marco comunitario de actuación en el ámbito de la política de aguas Off. J. Eur. Comm., L 327 (2000), p. 1 22.12.2000.
4. Anon Reglamento (CE) 1185/2009 n.º del Parlamento Europeo y del Consejo, de 25 de noviembre de 2009 relativo a las estadísticas de plaguicidas Off. J. Eur. Comm., L324 (2009), p. 1 10.12.2009.
5. Anon Directiva 2009/128 / CE del Parlamento Europeo y de 21 de octubre de 2009, por un marco de actuación comunitaria para conseguir un uso sostenible de los plaguicidas Off. J. Eur. Comm., L309 (2009), p. 71 24.11.2009.
6. Rosero-Moreano M., et al. 2014. J. Sep. Sci., 37, 3, 272-280.
7. Tolosa I., Readman JW. Anal. Chim. Acta, 335 (1996), p. 267.
8. Matisová E., Kakalikova L., Lesko J., de Zeeuw J., J. Chromatogr. A, 754 (1996), p. 445.
9. Oliveira, E. et al, Talanta 127 (2014) 59–67.
10. Ugland HG., Krogh M., Rasmussen KE., J. Chromatogr. B, 749 (2000), p. 85.
11. Jiang X., Lee HK., Anal. Chem., 76 (2004), 5591-5596.
12. Ho TS., Pedersen-Bjergaard S., Rasmussen K.E., J. Chromatogr. A, 963 (2002), p. 3.
13. Shen G., Lee HK. Anal. Chem., 74 (2002), p. 648.
14. Liu W, Zhang L, (et. al) J. Chromatogr. A, 1233 (2012), P. 1-7.
15. Rosero-Moreano M., et al 2014. J. Sep. Sci submitted December 31th 2014 (Special issue Colacro 2104 Latin American Congress of chromatography and related techniques).
16. Socas-Rodríguez, B., et al 2014. Food Chemistry 149 (2014) 319–325.
17. Valle de Bairros, A. et al 2014. Forensic Science International 243 (2014) 47–54.
18. Sharon, Y.Talanta 126 (2014) 163–169.
19. Kubáˇn, P. Boˇcek, P. Analytica Chimica Acta 787 (2013) 10– 23.
20. Arjomandi-Behzad, L. Yamini, Y. Talanta126(2014)73–81.
21. Cooper, K. Jankhaikhot, N. Journal of Chromatography A, 1358 (2014) 20–28.
22. Xu, X. Liang, F. et al. Analytica Chimica Acta 790 (2013) 39– 46.