http://dx.doi.org/10.4322/sc.2015.011

 

Restricted access material as sorbent for in-tube-LC-UV to determine sulfonamides in milk samples

Jardim, Valeria Cristina; Salami, Fernanda Helena; Chaves, Andrea R.; Queiroz, Maria Eugênia C.

Palavras-chave: HPLC, in-tube SPME, milk sample, restricted access material (RAM).

Resumo: The capillary packed with restricted-access materials (RAM) was developed for intube solid-phase microextraction and liquid chromatography analysis (in-tube SPME/LC-UV) for the determination of sulfonamides (sulfathiazole, sulfamethazine and sulfadimethoxine) in milk samples. The in-tube SPME variables (sample pH, diluted diluted sample volume, and desorption procedure) were optimized to improve the sensitivity of the method and short the analysis time. The proposed method presented a linear range from 30 ng mL–1 (limit of quantification – LOQ) to 150 ng mL-1, with the correlation coefficients (r) above 0.998. The accuracy values were adequate for all the sulfonamides (> 85%), and the interassay precision data presented coefficient of variation lower than 13%. Based on analytical validation results, the in-tube SPME/LC-UV method was a promising alternative for the determination of sulfonamides in milk samples.


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