http://dx.doi.org/10.4322/sc.2016.007

 

Determination of nine fungicides in orange juice by ultra performance liquid chromatography coupled to tandem mass spectrometry and mini-Luke extraction

Reichert, Jaqueline F.; Pizzutti, Ionara R.; Dias, Jonatan V.; Cardoso, Carmem D.

Palavras-chave: fungicides, mini-Luke, orange juice, UPLC-MS/MS.

Resumo: Brazil is the largest producer of orange juice, however, many of the pesticides allowed in citrus in Brazil are not allowed by importing countries. In this study mini-Luke extraction and ultra performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were used for the determination of nine fungicide pesticides in orange juice, all allowed in Brazil. Method linearity was studied in the range of 0.1 to 100 ng mL-1. Recovery and relative standard deviation (RSD%) were within the expected range (70 to 120% and ≤ 20%, respectively) for all fungicides, except for thiophanate-methyl. Quantification limit values were 10, 20 and 50 μg kg-1 for six, two and one fungicide studied, respectively, are equal to or lower than the values of the maximum residue limits established by the Brazilian, American and European legislation. The validated method was applied in the analysis of natural and processed orange juice (concentrate, diluted and integral), in a total of 20 samples. All samples showed contamination by piraclostrobin and trifloxistrobin; some samples showed contamination by carbendazim, tebuconazole and thiophanate-methyl.


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