Implementation of a dynamic extraction solvent assisted by sonication (DSASE) matrices erythroxylum coca leaf for subsequent analysis of cocaine by gas chromatography with flame ionization detector (GC-FID))
López, Ángela M.; Alvarez, Eliana; Cortés, Carolina; Rosero-Moreano, Milton; Taborda, Gonzalo; Garzón, William; Bernal, Hernando; Correa, Leonardo
Palavras-chave: Cocaine, gas chromatography with flame ionization detector (GC – FID), dynamic extraction solvent assisted by ultrasound (DSASE), Erythroxylum coca.
Resumo: The sample preparation technique called dynamic-assisted solvent extraction by sonication (DSASE) that uses sound waves to increase the efficiency of extraction of cocaine Erythroxylum coca leaves was implemented, evaluating different solvents and extraction time; for subsequent analysis by gas chromatography with flame ionization detector (GC-FID). The results show that the conditions for obtaining alkaloid are methanol: dichloromethane (20:80 v/v) with 25 min extraction at 50 ° C and 50 Hz; DSASE was coupled to the solid phase extraction (SPE) with conditions 1 mL of ethanol, 2 mL of sample, 1 mL of hexane and 1 mL of methanol: acetonitrile (20:80 v/v) for conditioning, loading, washing and elution respectively.