http://dx.doi.org/10.4322/sc.2017.015

 

Otimização de um método por CLAE-UV para análise de compostos fenolicos em Myrcia oblongata DC., Passiflora caerulea L. e Equisetum giganteum L.

Agostini, Fabiana; Michelon, Francine da Motta; Gomes, Vanessa Amanda Alves; Bertolazzi, Sabrina; Moura, Joséli Schwambach Sidnei

Palavras-chave: giant horstail, decoction, phytotherapy, guamirim, blue passion flower.

ResumoAmong the main active compounds present in plants used in traditional medicine are the phenolic compounds. However, there is a methodological restriction for qualification and simultaneous quantification of several representative of this class. Thus, this study aimed to optimize and validate a high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection method for the simultaneous determination of 14 phenolic compounds. In order to confirm the method efficiency, decoctions of Passiflora caerulea L. (blue passion fruit), Myrcia oblongata DC. (Guamirim) and Equisetum giganteum L. (giant horsetail) were used. There are few studies in the literature with these species. Nevertheless, these plants have been widely spread and used in folk medicine for their antioxidant characteristics. The method showed linearity, repeatability and accuracy. The correlation coefficients were between 0.9972 and 0.9999; detection limits ranged from 0.01 to 4.48 µg/mL and the quantitation limit from 0.04 to 13.58 µg/mL. In the tested decoctions, 09 compounds were identified, the major ones being vitexin and naringin in Guamirim; chlorogenic acid and naringin in horsetail; and vitexin and gallic acid in passion fruit. It was observed that Myrcia oblongata has a higher antioxidant potential with respect to the concentration of phenolics and their phenolic compounds individually investigated.


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