https://dx.doi.org/10.4322/sc.2017.018
Eletroforese capilar acoplada à espectrometria de massas (CE-MS): aspectos teóricos, práticos e aplicações no campo da metabolômica
Simionato, Ana Valéria Colnaghi; Santos, Fábio Neves dos; Hernandes, Vinicius Veri
Palavras-chave: eletroforese capilar acoplada a espectrometria de massas, metabolômica, fundamentos, avanços, aplicações.
Resumo: Há três décadas, a eletroforese capilar foi acoplada pela primeira vez à espectrometria de massas. Desde então, o desenvolvimento de instrumentação e metodologias vem sendo aperfeiçoado e, impulsionado, principalmente, pelas aplicações no campo da metabolômica. As interfaces CE-MS são o centro dos recentes desenvolvimentos que tentam integrar ao máximo as vantagens da separação por eletroforese e da detecção e identificação pelos analisadores de espectrometria de massas, em uma única técnica de alta eficiência. Atualmente, CE-MS é considerada uma técnica em plena consolidação, devido o crescente número de usuários e grupos de pesquisas trabalhando com aplicações em diferentes áreas da ciência. Em particular, a metabolômica e suas aplicações em bioanalítica se destacam com o maior uso das diferentes técnicas e instrumentações de CE-MS em virtude da sua relevância para o estudo de doenças, nos diagnósticos e no entendimento dos mecanismos bioquímicos envolvidos nas patologias clínicas, mas também nas áreas de alimentos para segurança alimentar e valor nutricional, ambiental focado em poluentes e contaminantes emergentes. A abrangência das aplicações e a inserção em diferentes áreas da ciência revela a consolidação de CE-MS no campo da metabolômica.
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