Removal of sulfamethoxazol and trimethoprim using horizontal-flow anaerobic immobilized bioreactor
Martins, Giovana Silva; Luchiari, Natália da Costa; Lamarca, Rafaela Silva; Silva, Bianca Ferreira da; Gomes, Paulo Clairmont Feitosa de Lima
Palavras-chave: Horizontal-flow anaerobic immobilized bioreactor (HAIB), Liquid chromatography tandem-mass spectrometry (LC-MS/MS), sulfamethoxazol, trimethoprim.
Resumo: Sulfamethoxazole (SMTX) and trimethoprim (TMP) are antibiotics present in wastewater in a concentration range µg L−1 to ng L−1. The stability in aquatic environment characterizes them as potential risk to the environment which explains the interest of monitoring environmental matrices. The development of simple analytical method to monitor antibiotics in wastewater is essential to evaluate new treatment technologies. The aim of this paper is to evaluate the removal efficiency of sulfamethoxazol and trimethoprim using a horizontal-flow anaerobic immobilized bioreactor (HAIB). A liquid chromatography/tandem mass spectrometry (LC-MS/ MS) method was developed using column-switching online solid-phase extraction as sample preparation procedure to monitor influent and effluent samples. LC-MS /MS were performed in positive mode (ESI +) and selected-reaction monitoring mode (SRM). The total analysis time was 13 minutes integrating the sample preparation and chromatographic run. Injection volume of 100 μL was sufficient to sample extraction. This method presented precision, linearity, quantification and detection limit for simultaneous detection of very low concentrations (ng L-1) from HAIB bioreactor influent and effluent. The precision intra-days showed values lower than 5%, linearity (75-500 ng L-1) and quantification limit (LOQ) of 75 ng L -1 for both antibiotics to the influent and effluent samples from the bioreactor.
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