A Cromatografia líquida moderna e a espectrometria de massas: finalmente “compatíveis”?
Lanças, Fernando M.
Palavras-chave: Liquid Chromatography, HPLC, Mass Spectrometry, LC/MS.
Resumo: High Performance Liquid Chromatography (HPLC) is one of the most utilized analytical techniques for determination of non volatile and thermally unstable compounds. Although being a separation technique, HPLC requires a confirmatory technique when qualitative analysis is being performed. Amongst the several available complimentary techniques, Mass Spectrometry is the one that supplies the structural information required to complement the HPLC separation; by coupling these two techniques, a powerful tool become available for qualitative and quantitative analysis: LC/MS (Liquid Chromatography-Mass Spectrometry). This paper presents in a critical and detailed description the fundamentals of the LC/MS technique.
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