¿Cuántos componentes hay en una mezcla? Respuesta cromatográfica

Stashenko, Elena E.; Martínez, Jairo R.

Palavras-chave: GC, GCxGC, Detection systems, Swinglea glutinosa, monocrotaline.

Resumo: The number of components determination in a complex mixture of substances is a fundamental problem for which various solutions with different accuracy may be applied, as a function of decisions taken along the analytical process, starting from sample preparation, following to the chromatographic technique, the injection mode, the chromatographic column, and the detection system employed. This article presents some examples to illustrate the incidence of several of these parameters on the definition of how many components are really present in a complex mixture.

Referências Bibliográficas

1. R. Grob. Modern practice of gas chromatography. Third Ed. Wiley, New York, 1999, 888pp.
2. J. Baker. Mass spectrometry. Second Ed. John Wiley & Sons, New York, 1999, 509 pp.
3. D. Barcelo. TrAC, Trends Anal. Chem., 27, 805 (2008).
4. K. Robards, P. Haddad, P. Jackson. Principles and practice of modern chromatographic methods. Academic Press, New York, 1994, 495 pp.
5. M. Doemoetoerova, E. Matisova. J. Chromatogr. A, 1207, 1 (2008).
6. B. d’Acampora Zellner, C. Bicchi, P. Dugo, P. Rubiolo, G. Dugo, L. Mondello. Flav. Fragr. J., 23, 297 (2008).
7. S. Ahuja. Chromatography and separation science. Academic Press, New York, 2003, 250 pp.
8. N. Jakubowska, B. Zygmunt, Z. Polkowska, B. Zabiegala, J. Namiesnik. J. Chromatogr. A, 1216, 422 (2009).
9. S. Risticevic, V. Niri, D. Vuckovic, J. Pawliszyn. Anal. Bioanal. Chem., 393, 781 (2009).
10. D. Watson. Natural toxicants in food. CRC Press, Sheffield, England, 1998, 335 pp.
11. R. Sievers. Selective detectors. Environmental, industrial, and biomedical applications. John Wiley & Sons, New York, 1995, 261 pp.
12. H. Cortes, B. Winniford, J. Luong, M. Pursch. J. Sep. Sci. 32, 883 (2009).
13. L. Mondello, P. Tranchida, P. Dugo, G. Dugo. Mass Spectrom. Rev., 27, 101 (2008).
14. M. Adahchour, J. Beens, U. Brinkman. J. Chromatogr. A, 1186, 67 (2008).
15. P. Marriot. J. Sep. Sci., 27 357 (2004).