Sample preparation: a crucial step in GC-MS analysis

Stashenko, Elena E.; Martínez, Jairo R.

Palavras-chaveGC, GC-MS, sample preparation, extraction techniques

Resumo The importance of sample preparation in GC and GC-MS analyses is highlighted. Examples of chromatographic profiles are used to illustrate the consequences of certain sample preparation mistakes. The main steps of the chain of events that leads to a suitable extract are described, in the context of the chromatographic determination of target analytes. A brief revision is made of the most common extraction methods, their scope and application fields. The use of positive and negative blanks, as well as surrogate substances and other recommendations for recovery improvement, the prevention of cross contamination, and the achievement of extracts containing few interferences, are presented together with explanations about their convenience and effects on the final analytical results.

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