Determination of benzene in beverages by solid-phase microextraction and gas chromatography
Sanchez, André B.; Budziak, Dilma; Martendal, Edmar; Carasek, Eduardo
Palavras-chave: Benzene, beverages, SPME, multivariate optimization.
Resumo: In this study, a simple and fast method was developed for the analysis of benzene in beverage samples using headspace solid-phase microextraction coupled to gas chromatography (HS-SPME-GC). The influence of factors such as extraction temperature, extraction time and addition of sodium chloride on extraction yield was studied through a multivariate experimental design. Satisfactory recoveries (≥73%) and precision, calculated as relative standard deviations (RSD ≤ 9.1%), were obtained. The limit of detection (LOD) of 0.2 μg L–1 was achieved for benzene along with a wide linear range of concentration. The applicability of the proposed method was demonstrated for real samples including soft-drink, juice and tea samples. Since no matrix effects were observed, quantification could readily be carried out by external calibration with standards prepared in deionized water.
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