Basic concepts in capillary electrophoresis

Spudeit, Daniel Alfonso; Dolzan, Maressa Danielli; Micke, Gustavo Amadeu

Palavras-chave: Capillary Electrophoresis, electroosmotic flow, instrumentation, injection modes.

Resumo Historically, the Swedish chemist Arne Tiselius was the first to use Electrophoresis as a separation tool, applying in blood proteins. The Capillary Electrophoresis emerged after decades as an improved technique providing an increase in efficiency and reduced analysis time. Its applications are wide and the range is from small and simple molecules organic and inorganic, to large and complex, as protein molecules and nucleic acids. This peculiar versatility of Capillary Electrophoresis makes it a promising tool for several knowledge areas. The methodologies developed by this technique can be improved offline by optimization and simulation softwares, highlighting the Peakmaster® software. This present paper will further introduce Capillary Electrophoresis instrumental aspects, injection modes and detection, as well as peculiar phenomena of technique, as electroosmotic flow.

Referências Bibliográficas

1. Tavares MFM. Eletroforese Capilar: conceitos básicos. Química Nova 1996; 19(2):173.
2. Tagliaro F, Manetto G, Crivellente F, Smith FP. A brief introduction to capillary electrophoresis. Forensic Science International 1998; 92(2-3):75-88. http://
3. Jorgenson JW, Lukacs KD. Zone Electrophoresis in Open-Tubular Glass-Capillaries. Analytical Chemistry 1981; 53(8): 1298-1302. http://dx.doi. org/10.1021/ac00231a037
4. Micke GA. Otimização e simulação em eletroforese capilar [tese]. São Paulo: Instituto de Química, Universidade de São Paulo; 2004. 311 p.
5. Manaenkov OV, Sidorov AI, Sul’man EM. Rapid determination of amino acids by capillary electrophoresis without preliminary derivatization. Journal of Analytical Chemistry 2003; 58(10):979-82.
6. Prokhorova AF Shapovalova EN, Shpigun OA. Chiral analysis of pharmaceuticals by capillary electrophoresis using antibiotics as chiral selectors. Journal of Pharmaceutical and Biomedical Analysis 2010; 53(5):1170-9. PMid:20675089. http://
7. Franco M, Jasionowska R, Salvatore E. Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations. International journal of analytical chemistry 2012; 2012:592. http://dx.doi. org/10.1155/2012/592650
8. Chen GH, Sun J, Dai YJ, Dong M. Determination of nicotinyl pesticide residues in vegetables by micellar electrokinetic capillary chromatography with quantum dot indirect laser-induced fluorescence. Electrophoresis 2012. 33(14):2192. PMid:22821497.
9. Piovezan M, Costa ACO, Jager AV, Oliveira MAL, Micke GA. Development of a fast capillary electrophoresis method to determine inorganic cations in biodiesel samples. Analytica Chimica Acta 2010; 673(2):200-5. PMid:20599036. http://
10. Jiang CX, Armstrong DW. Use of CE for the determination of binding constants. Electrophoresis 2010; 31(1):17‑27. PMid:20039286.
11. El-Hady D, Kühne S, El-Maali N, Wätzig H. Precision in affinity capillary electrophoresis for drug-protein binding studies. Journal of Pharmaceutical and Biomedical Analysis 2010; 52(2): 232-41. PMid:20080373. http://
12. Gervasio APG, Lavorante AF, Moraes MCB, Giné MF, Miranda CES, Carrilho E. Eletroforese capilar acoplada àespectrometria com plasma: uma ferramenta eficiente para a especiação. Química Nova 2003; 26(1):65-74.
13. Landers JP. Handbook of capillary and microchip electrophoresis and associated microtechniques. 3rd ed. Boca Raton: CRC Press; 2008. vol. 21, 1567 p., 6 p. of plates.
14. Gaš B. High Performance Capillary Electrophoresis – A primer. Agilent Technologies; 2009. 182 p.
15. Zhang Y, Gomez FA. Multiple-step ligand injection affinity capillary electrophoresis for determining binding constants of ligands to receptors. Journal of Chromatography A 2000; 897(1-2):339.
16. Vitali L, Horst BL, Heller M, Fávere VT, Micke GA. Using multiple short-end injections to develop fast electrophoretic separations-Applications in iodide analysis. Journal of Chromatography A 2011; 1218(28):4586-91. PMid:21621785. http://
17. Geiser L, Rudaz S, Veuthey JL. Decreasing analysis time in capillary electrophoresis: Validation and comparison of quantitative performances in several approaches. Electrophoresis 2005; 26(12):2293- 2302.. PMid:15895467. elps.200410242
18. Simpson SL, Quirino JP, Terabe S. On-line sample preconcentration in capillary electrophoresis Fundamentalsand applications.Journal of Chromatography A 2008; 1184(1-2):504-41. PMid:18035364. http://dx.doi. org/10.1016/j.chroma.2007.11.001
19. Vitali L, Favere VT, Micke GA. A new method to determine biological sample volume by short end multiple injection capillary electrophoresis: Application in determination of nitrate and thiocyanate in human saliva. Journal of Chromatography A 2011; 1218(16):2327-33. PMid:21397240. http://
20. Lucy CA, MacDonald AM, Gulcev MD. Non-covalent capillary coatings for protein separations in capillary electrophoresis. Journal of Chromatography A 2008. 1184(1-2):81-105. PMid:18164023. http://
21. Kuhr WG, Monnig CA. Capillary Electrophoresis. Analytical Chemistry 1992; 64(12): R389-R407. http://
22. Monnig CA, Kennedy RT. Capillary Electrophoresis. Analytical Chemistry 1994; 66(12):R280-R314. http://
23. Gaš B, Coufal P, Jaroš M, Muzikář J, Jelínek I. Optimization of background electrolytes for capillary electrophoresis I. Mathematical and computational model. Journal of Chromatography A 2001; 905(1-2):269-79. S0021-9673(00)00983-3
24. Štědrý M, Jaroš M, Gaš B. Eigenmobilities in background electrolytes for capillary zone electrophoresis – I. System eigenpeaks and resonance in systems with strong electrolytes. Journal of Chromatography A 2002; 960(1-2):187-98. PMid:12150556. http://
25. Štědrý M, Jaroš M, Včeláková K, Gaš B. Eigenmobilities in background electrolytes for capillary zone electrophoresis: II. Eigenpeaks in univalent weak electrolytes. Electrophoresis 2003; 24(3):536-47. PMid:12569543.