Chromatographic techniques and the challenge for the determination of compounds in complex matrices: Report of a case study of the natural product piperlongumine

Marques, Lucas Maciel M.; Oliveira, Anderson Rodrigo M. de

Palavras-chave: HPLC, UHPLC, GC, natural products.

ResumoThe chemical diversity of the natural products from plants represents a challenge for analytical methods of separation and detection. It is difficult to employ a single procedure to analise all compounds. Currently, chromatographic methods such as HPLC, GC and UHPLC have played an important role on the separation efficiency. The advantage arising from each one will depend on the experimental requirements. In this study, it will be analyzed the advantages and disavantages of these chromatographic techniques for determination of piperlongumine, as well as the resulting metabolites from the in vitro metabolism performed by rat liver microsomes. Regarding to the comparison, it is concluded that the techniques do not compete with them, unlike; they are complementary to each other.

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