GC/FID and GC/MS chemical composition analysis and antioxidant activity study of volatile secondary metabolites isolated by different methods from Satureja viminea L., grown in Colombia

Stashenko, Elena E.; Gutiérrez-Avella, Dora M.; Martínez, Jairo René; Manrique-López, Diana Lisseth

Palavras-chave: Satureja viminea L., essential oil, MWHD, SDE, SFE, HS-SPME, GC/FID, GC/MS, antioxidant activity.

Resumo: Volatile secondary metabolites from Satureja viminea L were extracted by microwave-assisted hydro-distillation, supercritical fluid (SFECO2 ) extraction, simultaneous distillation-solvent extraction (SDE), and headspace solid-phase micro-extraction (HS-SPME). Component identification was based on data from gas chromatography coupled to mass spectrometry (GC-MS) and their quantification employed gas chromatography with flame ionization detector (GC-FID), with n-tetradecane as internal standard. The oxygenated monoterpenes p-menth-3-en-8-ol (51,2%) and pulegone (17,8%) were the main components of the essential oil. In the extracts obtained by SDE and SFECO2 the major component, p-menth-3-en-8-ol was found at 58,0% and 38,9% abundances, respectively. The composition of the volatile fraction isolated with HS-SPME was dominated by p-menth-3-en-8-ol (46,2%), pulegone (11,2%), and pulegol (5,9%). The antioxidant activity of the essential oil determined by different methods (ORAC, ABTS+• and lipid peroxidation monitoring) was lower than those of the reference substances α-tocopherol (vitamin E) and 2,6-di-tert-butyl-4-methylphenol (BHT), except for that determined with the ORAC assay (1060±16 mmol Trolox® /g substance), which were almost twice those of the reference substances (vitamin E or BHT).

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