Caracterização das etapas de produção de cachaça por meio de perfis cromatográficos obtidos por cromatografia gasosa bidimensional abrangente
Cardeal, Zenilda de L.; Marriot, Philip
Palavras-chave: Comprehensive Two-Dimensional Gas Chromatography, Cachaça, Volatile Organic Compounds, SPME, GC×GC/TOFMS: Fingerprinting.
Resumo: Cachaça samples were studied by means of comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC/TOFMS) during the fermentation process, and after ageing in different wood materials. The analyses of the aroma compounds were performed after headspace-solid phase microextraction method (HS-SPME) using an 85 μm polyacrylate (PA) fiber. Fingerprint monitoring of the distillation process allowed the easy determination of the turning points of the process and high resolution comparison of cabeça (head), coração (core) and cauda (tail) fractions. The ageing process in different wood materials was well characterized through fingerprint similarity observations. In the absence of a suitable metric for expressing the overall similarity, here we use visual and retention time comparison to identify coincident peaks and those that differ between samples. For quality control purposes, a simple observation of the contour plots can thus allow the identification of the type of wood used in the ageing process, and the process of ageing, without further statistical treatment or peak identification. In this manner, the most discriminating peaks among different mixtures were readily identified, that is to say, unique compounds were identified in each stage of the distillation process. Approximate first dimension linear retention indices (I) for these identified compounds were calculated in a bi-dimensional polar/non-polar column set in the GC×GC experiment, and were used in conjunction with mass spectral library searching for tentative identification. Along the progression of the distillation process, 70 compounds appear to visually discriminate between samples and their retention indices are indicated, presenting good correlation with literature data.
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