Separation of steroids by temperature-programmed capillary liquid chromatography using packed fused-silica column

Monteiroa, Alessandra Maffei; Coutinhoa, Lincoln Figueira Marins; Nazariob, Carlos Eduardo Domingues; Lanças, Fernando Mauro

Palavras-chave: capillary liquid chromatography, temperature programming, steroids.

Resumo: The miniaturization of liquid chromatography and the development of fused silica capillary packed columns have enable a fast and homogeneous heat transmission through the analytical column. Thus, the use of temperature programming instead of gradient of mobile phase becomes an alternative to decrease the analysis time. This manuscript reports on the applicability of an analytical method for the separation of five steroids by temperature programming capillary liquid chromatography. The influence of the temperature on analytes retention time was investigated and discussed. The steroids were separated by a lab-made capillary column (RP-18) under isocratic elution. The temperature programming allowed to reduce twice the analysis time and diminished the band broadening of the most retained compound in comparison with isothermal analysis.

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